Plant Biotechnolog
Bsc 6th sem 2023 under dibrugarh University
2. Write briefly on any five of the following:
(a) Cryopreservation
(b) Recombinant DNA
(c) Phagemid
(d) Shuttle vector
(e) Golden rice
3. Write short notes on any four of the 5×4-20
following:
(a) Composition of MS medium
(b) Role of growth regulators in tissue culture
(c) Biological role of restriction enzyme
(d) Properties of a good cloning vector
(e) Lambda phaget issue culture media
4. Define transgenic crop. Discuss about thea pproaches to improve quality traits in
transgenic crops.
Or
Describe about the electroporation and microprojectile bombardment method of gene transfer.
5. Describe the method of protoplast isolation. Also discuss two strategies used for the selection of hybrid protoplast.
5+5=10
От
What do you mean by gene cloning? Discuss
in detail any two methods of construction of 2+8=10
genomic library.
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